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KMID : 1132720030010020108
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Genomics & Informatics
2003 Volume.1 No. 2 p.108 ~ p.112
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Combined Genome Mapping of RFLP-AFLP-SSR in Pepper
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Lee Je-Min
Kim Byung-Dong
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Abstract
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We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific $F_2$ population of 107 plants with 320 RFLP, 136 AFLP, and 46 SSR markers. The resulting linkage map consists of 15 linkage groups covering 1,720 cM with an average map distance of 3.7 cM between framework markers. Most RFLP markers ($80\%$) were pepper-derived clones and these markers were evenly distributed all over the genome. Genes for defense and biosynthesis of carotenoids and capsaicinoids were mapped on this linkage map. By using 30 primer combinations, AFLP markers were generated in the $F_2$ population. For development of SSR markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. This combined map provides a starting point for high-resolution QTL analysis, gene isolation, and molecular breeding.
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KEYWORD
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AFLP, Capsicum, mapping, RFLP, SSR
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